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  5. Thermo Scientific™ MIO Medium

Thermo Scientific™ MIO Medium

Catalog No. R061364
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Quantity:
20 x 5 mL
100 x 5 mL
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This item is not returnable. View return policy

Description

Differentiate enteric gram-negative bacilli on the basis of motility, production of indole, and ornithine decarboxylase using semi-solid MIO Medium (Motility Indole Ornithine Medium).

Differentiate enteric gram-negative bacilli on the basis of motility, production of indole, and ornithine decarboxylase with Thermo Scientific™ Remel™ MIO Medium (Motility Indole Ornithine Medium).

In 1970, Ederer and Clark developed MIO Medium (Motility Indole Ornithine Medium) to identify enteric gram-negative bacilli1. MIO Medium combines three test into a single tube to facilitate inoculation and identification of Enterobacteriaciae. Oberhofer and Hajkowski further investigated the use of MIO Medium for non-lactose fermenting, Klebsiella-Enterobacter-Serratia group of organisms2.

Use MIO Medium for differentiation of gram-negative bacilli on the basis of motility, production of indole, and ornithine decarboxylase.

  • Easy-to-differentiate: Based on motility, production of indole, and ornithine decarboxylase.
  • Ready-to-use: Convenience of prepared media.

Enteric gram-negative bacilli ferment dextrose and produce acid that changes the color of brom cresol purple indicator to yellow and also stimulates enzyme activity. Organisms that possess the enzyme ornithine decarboxylase convert ornithine to putrescine (alkaline) causing the pH indicator to revert to purple. The enzyme tryptophanase produced by some enteric, gram-negative bacilli degrades tryptophan present in the medium to form indole. This reaction is detected by adding Kovacs’ reagent to the surface of the medium. Indole combines with Kovacs’ reagent to form a red complex.

MIO Medium (Motility Indole Ornithine Medium) contains peptone and tryptone that supply nitrogenous compounds and amino acids necessary for the growth of enteric gram-negative bacilli. Yeast extract acts as the source of B-complex vitamins and dextrose acts as an energy source. Agar is added to enable detection of motility. Brom cresol purple is the pH indicator.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

TRUSTED_SUSTAINABILITY

Specifications

Certifications/Compliance Industrial Reference: BAM.
Description MIO Medium (Motility Indole Ornithine Medium)
Format 15mm x 103mm Tube
Product Type Microbiology Media
Quantity 100 x 5 mL
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