Thermo Scientific™ Tergitol 7 Agar w/TTC

Catalog Number	Mfr. No.	dimFormat
R01875	Thermo Scientific™ R01875
R01874	Thermo Scientific™ R01874

Description

Selectively isolate and differentiate coliform organisms with Thermo Scientific™ Remel™ Tergitol 7 Agar w/TTC. The use of tergitol (sodium heptadecyl sulfate) as a selective agent was first proposed by Pollard in 1946¹. Tergitol 7 Agar was later developed by Chapman for selective isolation and increased recovery of the coliform organisms^2,3. Later Tergitol 7 Agar was modified by addition of triphenyltetrazolium chloride (TTC) to facilitate early recognition of Escherichia coli and Enterobacter aerogenes within 6-10 hours of incubation.

Tergitol 7 Agar w/TTC is recommended for the selective isolation and differentiation of the coliform groups. Microorganisms capable of fermenting lactose produce yellow colonies on Tergitol 7 agar due to reaction of brom thymol blue indicator. Non-lactose fermenters produce blue to green or purple colonies.

TTC incorporated into Tergitol 7 Agar is a rapid indicator of bacterial growth. Some coliforms reduce TTC to insoluble formazan resulting in red colonies with blue zones that appear as purple. E. coli and E. aerogenes do not reduce TTC and form yellow colonies with yellow zones.

• Ready-to-use – Convenience of prepared media.
• Selective – Tergitol is a selective agent, which inhibits the growth of the gram-positive organisms and Proteus
• Easy-to-interpret – E. coli and E. aerogenes do not reduce TTC and form yellow colonies with yellow zones.

Tergitol 7 Agar w/TTC contains yeast extract, casein and meat peptone that provide nitrogen, amino acids and peptides necessary for bacterial growth. Lactose serves as the source of fermentable carbohydrate.

Tergitol-7 acts as a selective agent that inhibits gram positive organisms and minimises the swarming of Proteus, allowing superior recovery of coliforms. Brom thymol blue is the pH indicator.

Tri-phenyltetrazolium chloride (TTC) allows earlier recognition and identification of Escherichia coli and Enterobacter aerogenes.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Description: Tergitol 7 Agar w/TTC
• Product Type: Agar