Thermo Scientific™ Urea Broth

Differentiate Enterobacteriaceae based on urea hydrolysis using ready-to-use liquid medium Urea Broth (Stuart’s).

Supplier:  Thermo Scientific™ R065230

Catalog No. R065230

Description

Differentiate genera within the Enterobacteriaceae on the basis of urease production with Thermo Scientific™ Remel™ Urea Broth (Stuart’s). Urea Broth (Stuart’s) was developed by Stuart et al. to differentiate Proteus from other genera within the Enterobacteriaceae^1,2. This medium is designed such that it detects urease activity in organisms which produce ammonia in a highly buffered medium with limited nutrients. Such organisms include Proteus spp., Morganella morganii, Providencia rettgeri, and urease-positive Providencia stuartii³.

Urea Broth (Stuart’s) is a liquid medium used to detect microorganisms on the basis of urease production. It is a medium with high buffering capacity and limited nutrients. Other genera within the Enterobacteriaceae which hydrolyze urea, will not do so in this medium.

• Ready to use – Liquid medium.
• Easy to interpret – An intense pink-red color throughout the broth indicates a positive test. No color change, the broth remains yellow-orange in the case of a negative test.

This medium contains yeast extract that provides essential growth factors required by strong urease-positive organims such as Proteus spp., M. morganii, and some species of Providencia. Urea is the substrate that can be hydrolyzed. Disodium and monopotassium phosphates provide the buffering system. Phenol red acts as the pH indicator.

Organisms which possess the urease enzyme hydrolyze urea and produce an alkaline by-product (ammonia) which is detected when the phenol red indicator changes color from yellow-orange to pink.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Description: Urea Broth
• Format: 15mm x 103mm Tube
• Product Type: Microbiology Media
• Quantity: 100 x 2 mL