Invitrogen™ Renilla luciferase Recombinant Rabbit Monoclonal Antibody (SR07-830)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA532013

Catalog No. PIMA532013

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Renilla luciferase (RLuc), derived from the sea pansy Renilla reniformis, is a widely-used reporter gene in molecular biology for in vitro and in vivo assays, including gene expression studies and small animal imaging. The luciferase enzyme, approximately 37 kDa in size, belongs to the alpha/beta hydrolase family and operates via a catalytic mechanism involving the oxidative decarboxylation of its substrate, coelenterazine, in the presence of molecular oxygen, resulting in the emission of blue light. The gene encoding Renilla luciferase has been isolated and characterized, with its cDNA available for various applications. Structurally, RLuc contains a hydrolase fold akin to that of bacterial dehalogenases, suggesting an evolutionary relationship. Despite extensive utilization, the exact details of its active site and catalytic mechanism continue to be explored. The bioluminescent property of RLuc is particularly useful for generating a detectable signal in various biological assays, enhancing its utility as a biosensor.

Specifications

• Antigen: Renilla luciferase
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Renilla
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: SR07-830
• Conjugate: Unconjugated
• Gene Alias: Renilla-luciferin 2-monooxygenase
• Immunogen: Recombinant protein within Sea pansy Renilla Luciferase aa 1-96.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid