Gibco™ Residual Protein TrypLE™ ELISA Kit

Description

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

Specifications

• Assay Range: 0.78 to 50 ng/mL
• Assay Sensitivity: 0.032 ng/mL
• Content And Storage: • Pre-coated 96-well plate
  • Standard assay buffer concentrate
  • HRP-conjugated detection antibody
  • Wash buffer concentrate
  • Chromogen stop solution
  • Adhesive plate covers
  
  Store at 2–8°C.
  
  
• Conjugate: Horseradish Peroxidase (HRP)
• Immunoassay Kit Format: Sandwich ELISA Kit
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 6.20%
• Intraassay CV: 7.20%
• Label or Dye: Horseradish Peroxidase (HRP)
• Protein Aliases: TrypLE, R-Protease
• Quantity: 96 Tests kit
• Shipping Condition: Wet Ice
• Target: TrypLE (R-Protease) concentration
• Total Assay Time: 1.5 hr.
• Format: Wash steps required

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.