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Gibco™ Residual Protein TrypLE™ ELISA Kit

Catalog No. A6533101
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96 Tests kit
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A6533101 96 Tests kit
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The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

Specifications

Assay Range 0.78 to 50 ng/mL
Assay Sensitivity 0.032 ng/mL
Content And Storage • Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.

Conjugate Horseradish Peroxidase (HRP)
Immunoassay Kit Format Sandwich ELISA Kit
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 6.20%
Intraassay CV 7.20%
Label or Dye Horseradish Peroxidase (HRP)
Protein Aliases TrypLE, R-Protease
Quantity 96 Tests kit
Shipping Condition Wet Ice
Target TrypLE (R-Protease) concentration
Total Assay Time 1.5 hr.
Format Wash steps required
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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