Invitrogen™ RFC1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA535965

Catalog No. PIPA535965

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (88%), Rat (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.

Specifications

• Antigen: RFC1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RFC1
• Gene Alias: 140kDa; A1; A1 140 kDa subunit; A1-P145; Activator 1 140 kDa subunit; activator 1 large subunit; Activator 1 subunit 1; Alp145; Differentiation-specific element-binding protein; DNA-binding protein PO-GA; Ibf-1; ISRE-binding protein; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1; replication factor C (activator 1) 1, 145kDa; replication factor C 1; replication factor C 140 kDa subunit; Replication factor C large subunit; replication factor C subunit 1; Replication factor C subunit 1-like protein; replication factor C, 140 kDa; replication factor C1; RFC; RF-C 140 kDa subunit; Rfc1; RFC140
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5981
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P35251
• Gene Symbols: RFC1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 798 and 1147 of RFC1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov