Invitrogen™ RFC5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA596912

Catalog No. PIPA596912

Description

Immunogen sequence: LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA; Positive Samples: HeLa, U-251MG, SKOV3, MCF-7, Mouse liver, Mouse spleen; Cellular Location: Nucleus.

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Specifications

• Antigen: RFC5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RFC5
• Gene Alias: 2610020K06Rik; 2610209F07Rik; 36.5kDa; 36kDa; A1 36 kDa subunit; activator 1 36 kDa subunit; Activator 1 subunit 5; MGC1155; Recc5; replication factor C (activator 1) 5; replication factor C (activator 1) 5, 36.5kDa; replication factor C 36 kDa subunit; replication factor C 5; replication factor C subunit 5; replication factor c subunit 5-like protein; replication factor C subunit RFC5; replication factor C, 36 kDa; replication factor C, 36-kDa subunit; RF-C 36 kDa subunit; RFC, 36.5 kD subunit; RFC36; Rfc5; rfc5 protein; Unknown (protein for MGC:128229); zgc:110313
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 304528, 5985, 72151
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.46 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P40937, Q9D0F6
• Gene Symbols: RFC5
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 of human RFC5 (NP_0313961).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov