Invitrogen™ RFP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1986

Catalog No. PA1986

Description

PA1-986 detects RFP tagged proteins. This antibody is specific for the RFP tag and does not detect GFP or GFP tagged proteins. PA1-986 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of RFP tagged proteins. PA1-986 non-specifically detects a 45 kDa protein from E. coli extracts. This non-specific cross-reactivity is not present in mammalian samples. PA1-986 immunogen is a synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag. This peptide (Cat. # PEP-260) is available for use in neutralization and control experiments.

Red Fluorescent Protein (RFP) is isolated from sea anemone (Discosoma sp, Uniprot ID: Q9U6Y8), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.

Specifications

• Antigen: RFP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: DsRed2; mRFP; red fluorescent; Red fluorescent protein; RFP; RFP tag
• Immunogen: Synthetic peptide corresponding to residues V(22) N G H E F E I E G E G E G R(36) of the RFP tag.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Tag

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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