Invitrogen™ RFWD3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA567393

Catalog No. PIPA567393

Description

Immunogen sequence: NHTIPASSLHS MTNFISGLQR LHGMLEFLRP SSSNHSVGPM RTRRRVSASR RARAGGSQRT DSARLRAPLD AYFQVSRTQP DLPATTYDSE TRNPVSE Highest antigen sequence identity to the following orthologs - mouse 60%, rat 26%.

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). [UniProt].

Specifications

• Antigen: RFWD3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RFWD3
• Gene Alias: BC027246; E3 ubiquitin-protein ligase RFWD3; Rfwd3; RGD1305243; ring finger and WD repeat domain 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; Rnf201
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 55159
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q6PCD5
• Gene Symbols: RFWD3
• Immunogen: Recombinant Human RFWD3. Recombinant protein control fragment (Product #RP-108058).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG