Invitrogen™ RGMA Monoclonal Antibody (275407)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA523977

Catalog No. PIMA523977

Description

In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human RGM-A and recombinant chicken RGM is observed. In Western blots, approximately 25% cross-reactivity with recombinant mouse RGM-C is observed. Reconstitute at 0.5 mg/mL in sterile PBS.

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Specifications

• Antigen: RGMA
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q6PCX7, Q8JG54, Q96B86
• Gene Symbols: RGMA
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse RGM-A Cys48-Gly421.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Chicken
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot
• Clone: 275407
• Conjugate: Unconjugated
• Gene: RGMA
• Gene Alias: BC059072; C230063O06; repulsive guidance molecule; repulsive guidance molecule A; repulsive guidance molecule family member a; RGM; RGM domain family member A; RGM domain family, member A; RGMA
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 244058, 395214, 56963
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized