RhoB, Mouse anti-Monkey, Porcine, Rat, Chicken, Human, Mouse, Rabbit, Hamster, Clone: 7F7, Millipore Sigma™

Catalog No.	Quantity
MABS199025	25 μL

Catalog No. MABS199025 Supplier MilliporeSigma Supplier No. MABS199025UL

Description

Mouse Monoclonal Antibody

Anti-RhoB, clone 7F7, Cat. No. MABS1990, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB in human and has been tested for use in ELISA and Western Blotting.

Rho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human. RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form. RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli. Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation. RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation. It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage. It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells. RhoB displays 83% homology with RhoA. However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies. RhoB participates in sorting and degradation of growth factors and cytokine receptors. RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120). RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.

Specifications

• Antigen: RhoB
• Applications: ELISA, Western Blot
• Classification: Monoclonal
• Clone: 7F7
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10μg of  MCF7 cell lysate. Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts   (Courtesy of   Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA). ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB   (Courtesy of   Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).  
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Rho-related GTP-binding protein RhoB;Rho cDNA clone 6;h6
• Gene Symbols: RHOB;ARH6;ARHB
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
• Purification Method: Protein G purified
• Quantity: 25 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Clone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
• Target Species: Monkey, Pig, Rat, Chicken, Human, Mouse, Rabbit, Hamster
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1