Tocris Bioscience™ Rhobo6 ECM Dye

Catalog No.	Quantity
88-767-6U	76 μg

Catalog No. 88-767-6U Supplier Tocris Bioscience™ Supplier No. 8876/76U

Description

Fluorogenic probe for labeling the extracellular matrix (ECM) in live tissues

Key information: Rhobo6 ECM Dye is a cell-impermeable fluorescent probe for labeling the extracellular matrix (ECM) in live tissues. It contains a phenylboronic acid group that binds reversibly to diols commonly found in ECM glycans resulting in a significant increase in fluorescence and a red shift in emission spectra. For optimal signal, we recommend using 561 nm excitation and a 575 nm long-pass emission filter. Rhobo6 staining is not compatible with samples that are chemically fixed or that otherwise exhibit compromised cellular membranes.

Used for: labeling the ECM in live tissues.

Application: fluorescent microscopy including 2P and STED microscopy. Enables wash-free visualization of ECM in vitro and in vivo and is suitable for long-term imaging studies.

Properties and Photophysical Data: Rhobo6 provides broad substrate profile, superior tissue penetration, and negligible photobleaching. Excitation and emission maxima (λ) measured in 2 M sorbitol to mimic binding, are 535 nm and 561 nm, respectively; quantum yield = 0.75; extinction coefficient = 97,500 M-1cm-1.

Please refer to this guide for further information on how to use this product.

Specifications

• Chemical Name or Material: (E)-2-(6-((2-Boronobenzyl)amino)-3-((2-boronobenzyl)iminio)-3H-xanthen-9-yl)-4-carboxybenzoate trifluoroacetate
• Quantity: 76 μg
• Target: Live-cell Imaging
• Molecular Formula: C35H28B2N2O9.CF3CO2H
• Purity: 85%