Learn More
Invitrogen™ Rhodopsin Chimeric Recombinant Mouse Monoclonal Antibody (Rho 1D4)
Mouse Recombinant Monoclonal Antibody
Supplier: Invitrogen™ MA547924
Description
Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
Specifications
Rhodopsin Chimeric | |
Recombinant Monoclonal | |
1 mg/mL | |
PBS with 0.02% ProClin 300 | |
P02699, P08100, P15409, P35359, P51489 | |
RHO | |
Bleached bovine rod outer segment (ROS). | |
200 μg | |
Primary | |
Human, Mouse, Rat, Bovine, Amphibian, Zebrafish | |
Antibody | |
IgG1 κ |
ELISA, Immunohistochemistry, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry | |
Rho 1D4 | |
Unconjugated | |
RHO | |
CSNBAD1; L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; Noerg1; OPN 2; Opn2; Ops; opsin 2; opsin 2, rod pigment; opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rh; RH1; rh1.1; rho; rho protein; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; RP4; seven transmembrane light receptor; unknown opsin-like protein; wu:fi06d11; zfo2; zfrho | |
Mouse | |
Protein A | |
RUO | |
212541, 24717, 30295, 509933, 6010 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |
Your input is important to us. Please complete this form to provide feedback related to the content on this product.