Invitrogen™ Rhodopsin Chimeric Recombinant Mouse Monoclonal Antibody (Rho 1D4)

Mouse Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA547924

Catalog No. PIMA547924

Description

Specificity: The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

Specifications

• Antigen: Rhodopsin Chimeric
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% ProClin 300
• Gene Accession No.: P02699, P08100, P15409, P35359, P51489
• Gene Symbols: RHO
• Immunogen: Bleached bovine rod outer segment (ROS).
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine, Amphibian, Zebrafish
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
• Clone: Rho 1D4
• Conjugate: Unconjugated
• Gene: RHO
• Gene Alias: CSNBAD1; L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; Noerg1; OPN 2; Opn2; Ops; opsin 2; opsin 2, rod pigment; opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rh; RH1; rh1.1; rho; rho protein; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; RP4; seven transmembrane light receptor; unknown opsin-like protein; wu:fi06d11; zfo2; zfrho
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 212541, 24717, 30295, 509933, 6010
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid