Rhodopsin Monoclonal Antibody (4D2), APC, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA545244

Catalog No. PIMA545244

Description

1 μg/mL of MA5-45244 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.∣Detects approximately 40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

Specifications

• Antigen: Rhodopsin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.15mM MES, 47.35mM phosphate with 1.033mM EDTA and no preservative; pH 7.4
• Gene Accession No.: 0
• Gene Symbols: RHO
• Immunogen: Bovine Rhodopsin
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Amphibian, Avian, Fish, Mammalia, Shark
• Form: Liquid

• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: 4D2
• Conjugate: APC
• Gene: RHO
• Gene Alias: CSNBAD1; L opsin; Long Wavelength Sensitive opsin; LWS opsin; MGC138309; MGC138311; Noerg1; OPN 2; Opn2; Ops; opsin 2; opsin 2, rod pigment; opsin-2; Red Opsin; retinal rod opsin pigment rh1.1; Rh; RH1; rh1.1; rho; rho protein; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); Rhodopsin (retinitis pigmentosa 4, autosomal dominant); rhodopsin-like-1.1 rod opsin; Rod Opsin; RP 4; RP4; seven transmembrane light receptor; unknown opsin-like protein; wu:fi06d11; zfo2; zfrho
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 103176745
• Content And Storage: 4° C
• Isotype: IgG1