Invitrogen™ RhoGAP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527426

Catalog No. PIPA527426

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, ARHGAP1-transfected 293T. Predicted reactivity: Mouse (95%), Rat (92%), Dog (96%), Rhesus Monkey (98%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases.

Specifications

• Antigen: RhoGAP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARHGAP1
• Gene Alias: ARHGAP1; ARHGAP5; B230365D05Rik; C76222; CDC42 GTPase-activating protein; CDC42GAP; GFI2; growth factor independent 2; GTPase-activating protein rhoGAP; GTPase-activating protein rhoOGAP; GTPase-activating protein, Rho, 1; LOC512817 protein; p100 RasGAP-associated p105 protein; p105 RhoGAP; p190-B; p190BRhoGAP; p50rhoGAP; p50-RhoGAP; Rho GTPase activating protein 1; Rho GTPase activating protein 5; rho GTPase-activating protein 1; Rho GTPase-activating protein 5; RHOGAP; RHOGAP1; RHOGAP5; rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; rho-type GTPase-activating protein 5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 228359, 392
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q07960, Q5FWK3
• Gene Symbols: ARHGAP1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ARHGAP1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG