Invitrogen™ RIG-I Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5110296

Catalog No. PIPA5110296

Description

Immunogen sequence: QTRGRGRARG SKCFLLTSNA GVIEKEQINM YKEKMMNDSI LRLQTWDEAV FREKILHIQT HEKFIRDSQE KPKPVPDKEN KKLLCRKCKA LACYTADVRV IEECHYTVLG DAFKECFVSR PHPKPKQFSS FEKRAKIFCA RQNCSHDWGI HVKYKTFEIP VIKIESFVVE DIATGVQTLY SKWKDFHFEK IPFDPAEMSK.

Retinoic acid-inducible gene I, RIG-I is a pattern recognition receptor (PRR) involved in the recognition of viral dsRNA. Along with MDA5, RIG-I detects viral dsRNA and activates the innate immune response. Both MDA5 and RIG-I are RNA helicases and they perform overlapping as well as distinct roles. RIG-I is activated by dsRNAs without a 5'-triphosphate end and short dsRNAs, whereas MDA5 is activated by long dsRNAs. Once activated, both proteins signal through IPS-1 activating transcription factors NF-kappaB and IRF-3 (1) and ultimately activating apoptosis, cytokine signaling, and inflammation. RIG-I is essential for signaling by influenza A, influenza B, human respiratory syncytial virus (3), paromyxoviruses, Japanese encephalitis virus, and West Nile virus. MicroRNA-146a has been implicated in feedback inhibition of RIG-I-dependant antiviral response by negatively regulating RIG-I targets TRAF6, IRAK1, and IRAK2. Recent evidence has implicated RIG-I in the detection of cytosolic DNA through RNA polymerase III activity.

Specifications

• Antigen: RIG-I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DDX58
• Gene Alias: 6430573D20Rik; C330021E21; Ddx58; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD/H box polypeptide RIG-I; DEAD-box protein 58; DEXD/H-box helicase 58; probable ATP-dependent RNA helicase DDX58; Retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; retinoic acid-inducible gene-I; RIG-1; RIGI; RIG-I; RIG-I-like receptor 1; RLR-1; RNA helicase RIG-I; SGMRT2
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 230073, 23586, 297989
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1.26 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O95786, Q6Q899
• Gene Symbols: DDX58
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 726-925 of human RIG-I/DDX58 (NP_055129.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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