Invitrogen™ RIP2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528333

Catalog No. PIPA528333

Description

Recommended positive controls: HeLa, 293T, A431, H1299, HeLaS3, HepG2, Raji. Predicted reactivity: Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Specifications

• Antigen: RIP2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RIPK2
• Gene Alias: 2210420D18Rik; CARD3; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAK; CCK; D4Bwg0615e; EC 2.7.11.1; GIG30; growth-in; growth-inhibiting gene 30; kinase RIPK2; receptor (TNFRSF)-interacting serine-threonine kinase 2; receptor interacting serine/threonine kinase 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; receptor-interacting serine-threonine kinase 2; RICK; RIP 2; RIP2; RIP-2; Ripk2; RIP-like; RIP-like-interacting CLARP kinase; Tyrosine-protein kinase RIPK2; UNQ277/PRO314/PRO34092; WUGSC:H_RG437L15.1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8767
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.96 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O43353
• Gene Symbols: RIPK2
• Immunogen: Recombinant fragment corresponding to a region within amino acids 207 and 527 of Human RIP2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov