MilliporeSigma™ RME-8, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABN1657

Catalog No. ABN1657

Description

DnaJ homolog subfamily C member 13 (UniProt O75165; also known as Required for receptor-mediated endocytosis 8, RME-8) is encoded by the DNAJC13 (also known as KIAA0678, PARK21, RME8) gene (Gene ID 23317) in human. Originally identified as a C. elegans protein involved in mediating endocytosis, , receptor-mediated endocytosis 8 (RME-8) is a member of the DNAJ family of proteins implicated in regulating protein folding through associated chaperones. RME-8 is shown to interact with Hsc70/HSP-1 and sorting nexin 1 (SNX-1), loss of RME-8, SNX-1 or Hsc70 affects retrograde trafficking at the early endosomes-TGN interface. In addition, RME-8 is demonstrated to coordinate the function of the WASH complex and the retromer SNX dimer. The pentameric WASH complex is comprised of WASH1, strumpellin, SWIP (strumpellin- and wash-interacting protein), FAM21, and CCDC53. RME-8 N-terminal J region (a.a. 1-453) involved in membrane association is found to be involved in establishing interaction with the first third of FAM21 tail domain. WASH complex is responsible for the generation of branched actin networks on endosomes and the creation of microdomains into which specific cargoes can be concentrated. Loss of RME-8 function results in a profound endosomal tubulation, more extensive than the endosomal tubulation phenotype resulted from loss of the WASH complex.

Specifications

• Antigen: RME-8
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNAJC13, KIAA0678, PARK21, RME8
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antibody detected shRNA-mediated cellular RME-8 knockwon by immunoblotting and fluorescent immunocytochemistry (Freeman, C.L., et al. (2014). J. Cell Sci. 127(Pt 9):2053-2070; Girard, M., and McPherson, P.S. (2008). FEBS Lett. 582(6):961-966; Girard, M., et al. J. Biol. Chem. 280(48):40135-40143).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% Sodium Azide and 50% glycerol.
• Gene Accession No.: O75165
• Immunogen: KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human RME-8.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_056083
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.