Invitrogen™ RNASE7 Monoclonal Antibody (CL0224)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524591

Catalog No. PIMA524591

Description

Immunogen sequence: KGMTSSQWFK IQHMQPSPQA CNSAMKNINK HTKRCKDLNT FLHEPFSSVA ATCQTPKIAC KNGDKNCHQS HGPVSLTMCK LTSGKYPNCR YKEKRQNKSY VVACKPPQKK DSQQFHLVPV HLDRV Highest antigen sequence identity to the following orthologs: Mouse - 54%, Rat - 56%. Binds to an epitope located within the peptide sequence KGMTSSQWFKIQHMQ as determined by overlapping synthetic peptides.

The RNASE7 gene encodes a ribonuclease enzyme characterized by its potent ribonucleolytic activity and antimicrobial properties. It is part of the RNase A superfamily, featuring eight conserved cysteines and a catalytic histidine-lysine-histidine triad. RNase 7 is expressed in various somatic tissues, including the liver, kidney, skeletal muscle, and heart, and is prominently found in the stratum corneum of human skin. Its role is crucial in innate immune defense, assisting in the high resistance of human skin against infections. The expression of RNASE7 can be significantly induced by pro-inflammatory cytokines such as interferon-?, interleukin 1β, and to a lesser extent by tumor necrosis factor-a. This induction highlights its role in immune responses. Beyond its antimicrobial activity, RNase 7 has been shown to play a role in translating self-nucleic acids into TLR9 activators, influencing the DNA-mediated activation of immune cells like keratinocytes. These functions underscore RNASE7's significance in both maintaining skin barrier function and modulating immune responses.

Specifications

• Antigen: RNASE7
• Classification: Monoclonal
• Concentration: 1.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9H1E1
• Gene Symbols: RNASE7
• Immunogen: Recombinant protein corresponding to Human RNASE7.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: CL0224
• Conjugate: Unconjugated
• Gene: RNASE7
• Gene Alias: RAE1; Ribonuclease 7; ribonuclease A family member 7; ribonuclease, RNase A family, 7; RNase 7; RNASE7; SAP-2; skin-derived antimicrobial protein 2; UNQ2516/PRO6006
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 84659
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid