Invitrogen™ RNF168 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531119

Catalog No. PIPA531119

Description

Recommended positive controls: PC-3, human RNF168-transfected 293T. Predicted reactivity: Chimpanzee (98%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.

Specifications

• Antigen: RNF168
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RNF168
• Gene Alias: 3110001H15Rik; E3 ubiquitin-protein ligase RNF168; FLJ35794; hRNF168; RING finger protein 168; ring finger protein 168, E3 ubiquitin protein ligase; ring fnger protein 168; RING-type E3 ubiquitin transferase RNF168; RNF168
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 165918
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8IYW5
• Gene Symbols: RNF168
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RNF168. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov