Invitrogen™ RNF20 Monoclonal Antibody (3C6E2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549255

Catalog No. PIMA549255

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human MOLT-4 whole cell, human 293T whole cell, human A431 whole cell, rat brain tissue, mouse brain tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

Specifications

• Antigen: RNF20
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4 mg trehalose and no preservative
• Gene Accession No.: Q5DTM8, Q5VTR2
• Gene Symbols: RNF20
• Immunogen: E.coli-derived human RNF20 recombinant protein (Position: E123-S389).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Western Blot
• Clone: 3C6E2
• Conjugate: Unconjugated
• Gene: RNF20
• Gene Alias: 4833430L21Rik; AW540162; BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1A; BRE1-A; C79397; E3 ubiquitin-protein ligase BRE1A; FLJ11189; FLJ20382; hBRE1; HNF3B; homolog of S. cerevisiae BRE1; hypothetical protein; KIAA2779; Kiaa4116; MGC129667; MGC129668; mKIAA4116; RCJMB04_5a23; RING finger protein 20; ring finger protein 20, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase BRE1A; Rnf20; TCF3B
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 109331, 313216, 56254
• Content And Storage: -20°C
• Form: Lyophilized