ROBO-1 Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-1798	Rockland Immunochemicals 600401692	100 μg
50-199-1799	Rockland Immunochemicals 600401692S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human ROBO-1 protein. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

Specifications

• Antigen: ROBO-1
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Canine, Human, Mouse, Rat
• Gene Accession No.: O55005, O89026, Q9Y6N7
• Gene ID (Entrez): 19876, 487690, 58946, 6091
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: AW494633; AW742721; Deleted in U twenty twenty; Dutt1; FLJ21882; Gm310; HGNC:10249; H-Robo-1; MGC131599; MGC133277; Robo1; roundabout 1; roundabout guidance receptor 1; Roundabout homolog 1; roundabout homolog 1 (Drosophila); roundabout, axon guidance receptor, homolog 1; roundabout, axon guidance receptor, homolog 1 (Drosophila); SAX3
• Gene Symbols: ROBO1
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: ROBO1