Invitrogen™ ROBO1 Polyclonal Antibody

Catalog No.	Quantity
PIPA5120344	100 μL

Catalog No. PIPA5120344 Supplier Invitrogen™ Supplier No. PA5120344

Description

Rabbit Polyclonal Antibody

Positive test controls include: 293T, U-251MG, HeLa, 293T, U-251MG, HeLa, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Cell projection, Single-pass type I membrane protein, axon. Immunogen sequence: AAVMQKTRPA KKLKHQPGHL RRETYTDDLP PPPVPPPAIK SPTAQSKTQL EVRPVVVPKL PSMDARTDRS SDRKGSSYKG REVLDGRQVV DMRTNPGDPR EAQEQQNDGK GRGNKAAKRD LPPAKTHLIQ EDILPYCRPT FPTSNNPRDP SSSSSMSSRG SGSRQREQAN VGRRNIAEMQ VLGGYERGED NNEELEETES.

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

Specifications

• Antigen: ROBO1
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.47 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene: ROBO1
• Gene Accession No.: O55005, O89026, Q9Y6N7
• Gene Alias: AW494633; AW742721; deleted in U twenty twenty; DUTT1; FLJ21882; Gm310; HGNC:10249; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout 1; roundabout guidance receptor 1; roundabout homolog 1; roundabout homolog 1 (Drosophila); roundabout, axon guidance receptor, homolog 1; roundabout, axon guidance receptor, homolog 1 (Drosophila); Roundabout1 protein; SAX3
• Gene Symbols: ROBO1
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1452-1651 of human ROBO1 (NP_002932.1).
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19876, 58946, 6091
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG