Invitrogen™ ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)

Catalog No.	Quantity
FEMA550631	100 μL

Catalog No. FEMA550631 Supplier Invitrogen™ Supplier No. MA550631

Description

Mouse Recombinant Monoclonal Antibody

Positive control: MCF-7 cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, human breast tissue, human placenta tissue. Predicted band size: 181 kDa Subcellular Location: Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane.

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

Specifications

• Antigen: ROBO1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: 10E2-R
• Concentration: 2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene: ROBO1
• Gene Accession No.: O55005, O89026, Q9Y6N7
• Gene Alias: AW494633; AW742721; deleted in U twenty twenty; DUTT1; FLJ21882; Gm310; HGNC:10249; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout 1; roundabout guidance receptor 1; roundabout homolog 1; roundabout homolog 1 (Drosophila); roundabout, axon guidance receptor, homolog 1; roundabout, axon guidance receptor, homolog 1 (Drosophila); Roundabout1 protein; SAX3
• Gene Symbols: ROBO1
• Host Species: Mouse
• Immunogen: Recombinant protein within Human ROBO1 aa 51-300.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19876, 58946, 6091
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1