Invitrogen™ ROBO3 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA547463

Catalog No. PIPA547463

Description

In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ROBO3 and less than 5% cross-reactivity with rhROBO2 and rhROBO4 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.

Specifications

• Antigen: ROBO3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Robo3
• Gene Alias: FLJ21044; HGPPS; HGPS; RBIG1; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; Rig1; Rig-1; ROBO3; Robo3a; Robo3b; roundabout guidance receptor 3; roundabout homolog 3; roundabout homolog 3 (Drosophila); roundabout, axon guidance receptor, homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); roundabout-like protein 3
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19649
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry (Frozen), Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9Z2I4
• Gene Symbols: Robo3
• Immunogen: Mouse myeloma cell line NS0-derived mouse ROBO3 Gly54-Ser545.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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