Invitrogen™ ROBO3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA592319

Catalog No. PIPA592319

Description

Immunogen sequence: EMTPPLQGPR ARFRKKPKAL PYRRENSPGD LPPPPLPPPE EEASWALELR AAGSMSSLER ERSGERKAVQ AVPLAAQRVL HPDEEAWLPY SRPSFLSRGQ GTSTCSTAGS NSSRGSSSSR GSRGPGRSRS RSQSRSQSQR PGQKRREEPR; Positive Samples: Mouse brain; Cellular Location: Membrane, Single-pass type I membrane protein.

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla.

Specifications

• Antigen: ROBO3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Robo3
• Gene Alias: FLJ21044; HGPPS; HGPS; RBIG1; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; Rig1; Rig-1; ROBO3; Robo3a; Robo3b; roundabout guidance receptor 3; roundabout homolog 3; roundabout homolog 3 (Drosophila); roundabout, axon guidance receptor, homolog 3; roundabout, axon guidance receptor, homolog 3 (Drosophila); roundabout-like protein 3
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19649
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.64 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q9Z2I4
• Gene Symbols: Robo3
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1237-1386 of human ROBO3 (NP_0717652).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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