Invitrogen™ ROCK1 (Cleaved Asp1113) Monoclonal Antibody (154C1465)

Catalog No.	Quantity
01-676-444	100 μg

Catalog No. 01-676-444 Supplier Invitrogen™ Supplier No. MA141031

Description

Mouse Monoclonal Antibody

This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for the detection of apoptosis. A suggested positive control is Jurkat cells treated with camptothecin or anti-Fas antibody to induce apoptosis. Cross reacts with Chimpanzee.

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

Specifications

• Antigen: ROCK1 (Cleaved Asp1113)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 154C1465
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.05% sodium azide
• Gene: ROCK1
• Gene Accession No.: O77819, P70335, Q13464, Q63644
• Gene Alias: 1110055K06Rik; Ac2-154; cAMP-dependent protein kinase ROCK-I; cePKA; corneal epithelial Rho-associated-ser/thr kinase; corneal epithelial Rho-associated-Ser/Thr kinase 1; HEBM1; liver regeneration-related protein LRRG199; MGC131603; MGC43611; p150 RhoA-binding kinase ROK beta; p160 ROCK-1; p160ROCK; PRO0435; renal carcinoma antigen NY-REN-35; Rho associated coiled-coil containing protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; Rho-associated coiled-coil forming kinase 1; Rho-associated kinase beta; rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK1; Rock-I
• Gene Symbols: ROCK1
• Host Species: Mouse
• Immunogen: Synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100009220, 19877, 480181, 6093, 81762
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov