Invitrogen™ ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor™ 610, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-4863	100 μg
50-112-4862	25 μg

Catalog No. 50-112-4863 Supplier Invitrogen™ Supplier No. 61698182

Description

Rat Monoclonal Antibody

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs. The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms. Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular protein under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test.

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas. ROR gammat t (RORgt) is an isoform of RORC.

Specifications

• Antigen: ROR gamma (t)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: B2D
• Concentration: 0.2 mg/mL
• Conjugate: PE-eFluor 610
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Rorc
• Gene Accession No.: P51450
• Gene Alias: EGK_01272; IMD42; MGC129539; NR1F3; nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F member 3; RAR related orphan receptor C; RAR-related orphan nuclear receptor variant 2; RAR-related orphan receptor C; RAR-related orphan receptor C, isoform a; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; Rorc; Rorg; RORgamma; RORgt; RP11-98D18.11-001; RZRG; RZR-GAMMA; Thor; Thymus orphan receptor; TOR
• Gene Symbols: Rorc
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19885
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.