ROR1 Mouse anti-Human, Brilliant Violet 510, Clone: 4A5, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743013

Catalog No. BDB743013

Description

The 4A5 monoclonal antibody binds to the glycosylated type I membrane protein ROR1 (Receptor-tyrosine-kinase-like Orphan Receptor 1). The ROR1 and ROR2 related proteins are highly conserved evolutionarily and are primarily expressed during embryogenesis. ROR1 is not expressed on normal peripheral blood lymphocytes. ROR1 is similar to tropomyosin receptor kinase (Trk family) neurotropic receptors and shares a cysteine-rich domain with Frizzled receptors for Wnt-family signaling proteins, which are involved in the regulation of embryogenesis and carcinogenesis. Furthermore, there is mounting evidence that aberrant ROR1 expression contributes to human malignancy.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: ROR1
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Neurotrophic tyrosine kinase, receptor-related 1, NTRKR1
• Immunogen: Human ROR1 cDNA
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human

• Clone: 4A5
• Conjugate: Brilliant Violet 510
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse BALB/c IgG_2b, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal