Invitrogen™ RPA4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA575738

Catalog No. PIPA575738

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB consisting of three subunits that play an important role in DNA replication, recombination and repair. RPA is one of the major damage- recognition structures involved in the early stage of nucleotide excision repair and may function in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. Widely expressed at low to intermediate levels, RPA 34 kDa subunit, which is also known as RPA4 (replication factor A protein 4), contains 261 amino acids, localizes to nucleus and is preferentially expressed in mucosa of colon and placenta.

Specifications

• Antigen: RPA4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RPA4
• Gene Alias: HSU24186; MGC120333; MGC120334; Replication factor A protein 4; replication protein A 30 kDa subunit; replication protein A complex 34 kd subunit homolog Rpa4; replication protein A4; replication protein A4, 30kDa; replication protein A4, 34kDa; RF-A protein 4; RP-A p30; RPA4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29935
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q13156
• Gene Symbols: RPA4
• Immunogen: Synthetic peptide corresponding to residues in Human RPA4.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG