Invitrogen™ RPH3A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1774

Catalog No. PA1774

Description

PA1-774 detects rabphilin 3A from rat tissues. PA1-774 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~75 kDa protein representing rabphilin 3A from rat brain whole protein extract. PA1-774 immunogen is a synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A. This sequence is completely conserved between rat and mouse rabphilin 3A. PA1-774 immunizing peptide (Cat. # PEP-102) is available for use in neutralization and control experiments.

The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.

Specifications

• Antigen: RPH3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RPH3A
• Gene Alias: 2900002P20Rik; AU022689; AW108370; Exophilin 1; Exophilin1; Exophilin-1; KIAA0985; rabphilin 3A; rabphilin 3A homolog; rabphilin-3A; RPH3A
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 171039, 19894
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P47708, P47709
• Gene Symbols: RPH3A
• Immunogen: Synthetic peptide corresponding to residues M(1) T D T V V N R W M Y P G D G P L Q(18) C of rat Rabphilin 3A.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG