Invitrogen™ RPH3A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA535960

Catalog No. PIPA535960

Description

Recommended positive controls: mouse brain, rat brain. Predicted reactivity: Mouse (88%), Rat (88%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B, and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.

Specifications

• Antigen: RPH3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RPH3A
• Gene Alias: 2900002P20Rik; AU022689; AW108370; Exophilin 1; Exophilin1; Exophilin-1; KIAA0985; rabphilin 3A; rabphilin 3A homolog; rabphilin-3A; RPH3A
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 171039, 19894, 22895
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P47708, P47709, Q9Y2J0
• Gene Symbols: RPH3A
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Rabphilin 3A. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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