Cytiva rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Catalog Number	Mfr. No.	Binding Capacity	Quantity
45-002-972	Cytiva 17127902	50 mg Human lgG/mL	25 mL
45-002-971	Cytiva 17127901	50 mg Human lgG/mL medium2	5 mL
45-002-973	Cytiva 17127903	50 mg Human lgG/mL medium2	200 mL

Description

• Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
• High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
• No mammalian components involved during manufacturing process
• Matrix: 4% cross-linked agarose
• Average particle size: 90μm (d50V)
• Ligand: 6mg rProtein A/mL medium (E. coli)
• Binding capacity: ∽50mg human IgG/mL media
• pH stability working range: 3 to 10
• Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

Specifications

• Particle Size: 60 - 165 µm
• Flow Rate: 150 to 250 cm/Hoµr
• Height (English) Bed: 9.84 in.
• Ligand Type: Recombinant Protein A (E. coli)
• Max. Pressure: 100 kPa
• Stability: Stable in aqueous Buffers commonly used in Protein A Chromatography
• Storage Requirements: 2°C to 8°C

• Product Type: rProtein A Sepharose fast flow media
• For Use With (Application): For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• pH Range: 2 to 9
• Storage Buffer: 20% Ethanol