Invitrogen™ RRAS Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528270

Catalog No. PIPA528270

Description

Recommended positive controls: H1299, HeLaS3, Molt-4, Raji, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (93%), Rat (91%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signal pathways control such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that control eukaryotic cell function by cycling between two interconvertible forms, a GDP-bound inactive form and a GTP-bound active form. The binding of GTP leads to a conformational change in the downstream effector-binding domain of the G-protein. The small GTPases are monomeric G-proteins with molecular masses over the range 20-30 kDa. Ras is attached to the cell membrane by prenylation. Mutations in the Ras family of proto-oncogenes (comprising H-Ras, N-Ras and K-Ras) are very common, being found in 20% to 30% of all human tumours. The frequency of RAS mutations is among the highest for any gene in human cancer. K-ras mutations occur frequently in non-small-cell lung, colorectal, and pancreatic carcinomas; H-ras mutations are common in bladder, kidney, and thyroid carcinomas; N-ras mutations are found in melanoma, hepatocellular carcinoma, and hematologic malignancies.

Specifications

• Antigen: RRAS
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RRAS
• Gene Alias: AI573426; Harvey rat sarcoma oncogene, subgroup R; Harvey rat sarcoma virus oncogene, subgroup R; oncogene RRAS; p23; ras family small GTP binding protein R-Ras; ras-related protein R-Ras; Ras-related protein R-RAS (P23); related RAS viral (r-ras) oncogene homolog; RRAS
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20130, 361568, 6237
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.63 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: D3Z8L7, P10301, P10833
• Gene Symbols: RRAS
• Immunogen: Recombinant fragment corresponding to a region within amino acids 11 and 218 of Human RRAS.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG