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RT-PCR: The Molecular Biology of HIV Replication



Unique, advanced-level demonstration of reverse transcriptase.
Supplier: Edvotek 335
Includes: All necessary solutions, buffers, chemicals and templates for six sets of reactions; complete instructions; background information and study questions
Requires :
- Horizontal gel electrophoresis apparatus
- Thermal cycler
- Hotplate
- Micropipetter
- Balance
- Water bath
- 250mL Flasks
- UV transilluminator
- Pipet pump or bulb
- All necessary safety equipment
- Distilled or deionized water
Description
Similar to HIV replication
- Reactions of the provided mRNA are reverse transcribed to double-stranded DNA
- Amplify the DNA using PCR in order to see the results of this reaction
Order Info
Supplies for six reactions
Specifications
Instructions, RNA template, primer mix, RT-PCR reaction beads, RNase free water, DNA size ladder, Agarose, electrophoresis Buffer, InstaStain™ Ethidium Bromide. Store at -20°C | |
Lyophilized | |
RT-PCR | |
RT-PCR kit | |
Classroom Experiment |
Gel Electrophoresis | |
Medium | |
30 to 90 min. | |
SYBR Green, FlashBlue Stain | |
RT-PCR |
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