Invitrogen™ RTCA Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA537049

Catalog No. PIPA537049

Description

This antibody detects endogenous protein at a molecular weight of 39 kDa. Purity is >95% by SDS-PAGE.

RTCD1 catalyzes the conversion of 3'-phosphate to a 2', 3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. There are two named isoforms.

Specifications

• Antigen: RTCA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RTCA
• Gene Alias: 2310009A18Rik; AI450277; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA terminal phosphate cyclase domain 1; RNA terminal phosphate cyclase domain-containing protein 1; RNA-3'-phosphate cyclase; RPC; Rpc1; RTC domain-containing protein 1; RTC1; RTCA; Rtcd1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 295395, 8634
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O00442
• Gene Symbols: RTCA
• Immunogen: Synthetic peptide corresponding to amino acids 321-366 of human RTCD1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat, Rat
• Product Type: Antibody
• Isotype: IgG