Invitrogen™ RTF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5110333

Catalog No. PIPA5110333

Description

Immunogen sequence: LAKKQPLKTS EVYSDDEEEE EDDKSSEKSD RSSRTSSSDE EEEKEEIPPK SQPVSLPEEL NRVRLSRHKL ERWCHMPFFA KTVTGCFVRI GIGNHNSKPV Y.

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

Specifications

• Antigen: RTF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RTF1
• Gene Alias: 2900005O08Rik; 6530416A09Rik; AI853581; AU043144; AW553985; cbp82; CG10955; CG10955-PA; CG10955-PB; Dmel\CG10955; Dmel_CG10955; dRtf1; gene trap locus 7; Gtl7; KIAA0252; ortholog of mouse gene trap locus 7; RNA polymerase-associated protein Rtf1; RNA polymerase-associated protein RTF1 homolog; Rtf1; RTF1 homolog, Paf1/RNA polymerase II complex component; Rtf1 Paf1/RNA polymerase II complex component homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog; Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae); Rtf1-PA; Rtf1-PB
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23168, 76246
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.57 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: A2AQ19, Q92541
• Gene Symbols: RTF1
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human RTF1 (NP_055953.3).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov