Invitrogen™ Rubicon Monoclonal Antibody (4F5B5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA548673

Catalog No. FERMA548673

Description

Rubicon Monoclonal Antibody for Western Blot, ELISA

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.

Specifications

• Antigen: Rubicon
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: Q92622
• Gene Symbols: RUBCN
• Immunogen: Purified recombinant fragment of human RUBCN (AA: 859-972) expressed in E. Coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Western Blot
• Clone: 4F5B5
• Conjugate: Unconjugated
• Gene: RUBCN
• Gene Alias: 1700021K19Rik; 5330403K09; baron; Beclin-1 associated RUN domain containing protein; KIAA0226; mKIAA0226; RGD1305422; RIKEN cDNA 1700021K19 gene; RUBCN; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cysteine-rich containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; SCAR15; similar to mKIAA0226 protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 9711
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid