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Invitrogen™ Rubicon Polyclonal Antibody
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Catalog No. PIPA577807
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100 μg
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PIPA577807 100 μg
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Catalog No. PIPA577807 Supplier Invitrogen™ Supplier No. PA577807
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Description

Rabbit Polyclonal Antibody

A 1:1,000 dilution of PA5-77807 was sufficient for detection of Rubicon in 15 μg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. Detects approximately 95 kDa.

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Rubicon
Applications Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
Gene RUBCN
Gene Accession No. Q92622
Gene Alias 1700021K19Rik; 5330403K09; baron; Beclin-1 associated RUN domain containing protein; KIAA0226; mKIAA0226; RGD1305422; RIKEN cDNA 1700021K19 gene; RUBCN; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cysteine-rich containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; SCAR15; similar to mKIAA0226 protein
Gene Symbols RUBCN
Host Species Rabbit
Immunogen Synthetic peptide from the N-terminal of Human Rubicon.
Purification Method Antigen affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 9711
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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