Invitrogen™ Rubicon Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA535969

Catalog No. PIPA535969

Description

Recommended positive controls: MDA-MB-231, NT2D1, human Rubicon-transfected 293T. Predicted reactivity: Mouse (92%), Pig (94%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.

Specifications

• Antigen: Rubicon
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RUBCN
• Gene Alias: 1700021K19Rik; 5330403K09; baron; Beclin-1 associated RUN domain containing protein; KIAA0226; mKIAA0226; RGD1305422; RIKEN cDNA 1700021K19 gene; RUBCN; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cysteine-rich containing protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN domain protein as Beclin 1-interacting and cysteine-rich containing; rundataxin; SCAR15; similar to mKIAA0226 protein
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 9711
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot
• Concentration: 0.48 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q92622
• Gene Symbols: RUBCN
• Immunogen: Recombinant fragment corresponding to a region within amino acids 677 and 972 of Rubicon.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG