Invitrogen™ RYBP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA519943

Catalog No. PIPA519943

Description

In Western blot applications, this antibody detects a band at ~32kDa. A suggested positive control is A549 cell lysate. PA5-19943 can be used with blocking peptide PEP-0068.

RYBP involves component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3.

Specifications

• Antigen: RYBP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rybp
• Gene Alias: 2410018J24Rik; AAP1; APAP-1; apoptin-associating protein 1; Death effector domain-associated factor; DEDAF; DED-associated factor; RING1 and YY1 binding protein; RING1 and YY1-binding protein; ring1 interactor RYBP; Rybp; YEAF1; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23429, 312603, 56353
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q8CCI5, Q8N488
• Gene Symbols: Rybp
• Immunogen: DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF. The immunogen is located within the last 50 amino acids of DEDAF.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG