Invitrogen™ S-Adenosyl-Homocysteine Monoclonal Antibody (3B3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549612

Catalog No. PIMA549612

Description

Antibody tested in indirect ELISA.

S-Adenosylhomocysteine (AdoHcy) is the immediate precursor of all of the homocysteine produced in the body. The reaction is catalyzed by S-adenosylhomocysteine hydrolase and is reversible with the equilibrium favoring formation of AdoHcy. In vivo, the reaction is driven in the direction of homocysteine formation by the action of the enzyme adenosine deaminase, which converts the second product of the S-adenosylhomocysteine hydrolase reaction, adenosine, to inosine. Except for methyl transfer from betaine and from methylcobalamin in the methionine synthase reaction, AdoHcy is the product of all methylation reactions that involve S-adenosylmethionine (AdoMet) as the methyl donor. Methylation is significant in epigenetic regulation of protein expression via DNA and histone methylation. The inhibition of these AdoMet-mediated processes by AdoHcy is a proven mechanism for metabolic alteration. Because the conversion of AdoHcy to homocysteine is reversible, with the equilibrium favoring the formation of AdoHcy, increases in plasma homocysteine are accompanied by an elevation of AdoHcy in most cases. Disturbances in the transmethylation pathway indicated by abnormal S-adenosylmethionine, S-adenosylhomocysteine or their ratio have been reported in many neurodegenerative diseases, such as dementia, depression or Parkinson's disease. (PMID: 18065573, 17892439). [National Center for Biotechnology Information. PubChem Compound Database; CID=439155].

Specifications

• Antigen: S-Adenosyl-Homocysteine
• Classification: Monoclonal
• Concentration: 5.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry, Lateral Flow, SDS-Page
• Clone: 3B3
• Conjugate: Unconjugated
• Gene Alias: AdoHcy; C14H20N6O5S; Formycinylhomocysteine; S-(5'-adenosyl)-L-homocysteine; S-adenosylhomocysteine; S-adenosyl-L-homocysteine
• Immunogen: Conjugate of SAH hapten and KLH.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid