Invitrogen™ S-arrestin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1731

Catalog No. PA1731

Description

PA1-731 detects recombinant bovine and sheep visual arrestin. PA1-731 has been successfully used in immunofluorescence and Western blot procedures. By Western blot this antibody detects an ~57 kDa protein representing recombinant bovine and sheep visual arrestin. This antibody also detects a lower molecular weight protein which could correspond to degradation product. The PA1-731 immunizing peptide corresponds to amino acid residues 2-18 from rat visual arrestin. This peptide (Cat. # PEP-155) is available for use in neutralization and control experiments.

S-arrestin, also known as retinal arrestin or visual arrestin, is part of a small gene family that includes four members. It primarily functions in the retina, specifically within rod and cone photoreceptors. S-arrestin plays a crucial role in phototransduction by participating in the inactivation of opsin, which is a key component in the visual signaling pathway. Upon light stimulation, activated opsin interacts with a G-protein-coupled receptor kinase (GRK), leading to its phosphorylation. S-arrestin then binds to phosphorylated opsin to terminate the G-protein signaling, hence contributing to the process of vision adaptation and regulation of neurotransmitter release at the photoreceptor-bipolar cell synapse. This interaction is vital for the proper functioning of the visual cycle, ensuring that 11-cis-retinal is regenerated through the retinoid cycle. Mutations in the S-arrestin gene, like other rhodopsin alleles, are linked to various retinal disorders. The gene's expression pattern is specific to the retina, underscoring its role in vision, and it is distinct from other members of the arrestin family like beta-arrestins which regulate G-protein-coupled receptors (GPCRs) outside of the retina.

Specifications

• Antigen: S-arrestin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SAG
• Gene Alias: 48 kDa protein; A930001K18Rik; Arr1; arrestin; arrestin 1; arrestin1; arrestin-1; DKFZp686D1084; DKFZp686I1383; Irbp; photoreceptor protein/alleged cGMP phosphodiesterase activation modulator; put. S-antigen C-terminus; retinal S antigen; retinal S-antigen; retinal S-antigen (48 KDa protein); rod arrestin; rod photoreceptor arrestin; RP47; Sag; S-AG; SAGMR; SANTI; S-antigen; S-antigen visual arrestin; S-antigen, retina and pineal gland (arrestin); S-antigen; retina and pineal gland (arrestin); S-arrestin; S-arrestin short form; visual arrestin 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 101105037, 20215, 25539, 280922
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P08168, P15887, P20443
• Gene Symbols: SAG
• Immunogen: Synthetic Peptide: C E(347) V A T E V P F R L M H P Q P E D(363).
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Mouse, Sheep, Rat
• Product Type: Antibody
• Isotype: IgG