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S100A8/A9 Antibody (SPM281), Novus Biologicals™
SDP

Catalog No. NBP24529601 Shop All R&D Systems Products
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NBP24529601 0.1 mg
NBP24529600 0.02 mg
NBP24529602 0.2 mg
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Catalog No. NBP24529601 Supplier Novus Biologicals Supplier No. NBP2452960.1MG

Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

S100A8/A9 Monoclonal specifically detects S100A8/A9 in Human, Mouse, Rat, Porcine, Bovine, Canine, Equine, Feline, Guinea Pig, Goat, Baboon, Monkey, Rabbit samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen S100A8/A9
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone SPM281
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, S100 calcium binding protein A8, S100A8
Gene Symbols S100A8
Host Species Mouse
Immunogen Affinity purified monocyte membrane preparation
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cancer
Primary or Secondary Primary
Gene ID (Entrez) 6279
Test Specificity Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer s cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Target Species Human, Mouse, Rat, Pig, Baboon, Canine, Equine, Feline, Guinea Pig, Goat, Monkey, Rabbit
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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