S100A9 Antibody (47-8D3 (DF3973)), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2449140.1MG

Catalog No. NBP24491401

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

S100A9 Monoclonal specifically detects S100A9 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: S100A9
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P06702
• Gene Symbols: S100A9
• Immunogen: Human peripheral blood monocyte components
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: 47-8D3
• Conjugate: Unconjugated
• Formulation: 10mM PBS with 0.05% Sodium Azide
• Gene Alias: CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)
• Host Species: Mouse
• Molecular Weight of Antigen: 14 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases
• Gene ID (Entrez): 6280
• Target Species: Human, Rat
• Form: Purified

For Research Use Only