S100A9 Antibody (CPT/1028), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24491200	Novus Biologicals NBP2449120.02MG	0.02 mg
NBP24491201	Novus Biologicals NBP2449120.1MG	0.1 mg
NBP24491202	Novus Biologicals NBP2449120.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunohistochemistry (Frozen) (Negative)

S100A9 Monoclonal specifically detects S100A9 in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: S100A9
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 6280
• Immunogen: Recombinant human Calprotectin protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 14 kDa

• Clone: CPT/1028
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)
• Gene Symbols: S100A9
• Isotype: IgM κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections.

For Research Use Only