Invitrogen™ SAP (SLAM-Associated Protein) Monoclonal Antibody (XLP-1D12), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-978-742	100 Tests

Catalog No. 50-978-742 Supplier Invitrogen™ Supplier No. 50978742

Description

Rat Monoclonal Antibody

Description: The monoclonal antibody XLP-1D12 reacts with human SLAM-associated protein (SAP), which is also known as SH2D1A. SAP is a 15-kDa SH2 domain-containing adaptor protein. Upon CD150/SLAM family receptor ligation, SAP recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in thymocytes, T cells, NK cells, and NKT cells. Studies have demonstrated a role for SAP in promoting follicular T helper cell differentiation during germinal center formation. In addition, this protein is involved in NKT cell development, Th1/Th2 differentiation, development of innate T cells, and regulation of NK cell and CD8+ T cell cytotoxicity. Mutations of the gene encoding SAP results in X-linked lymphoproliferative (XLP) syndrome, which is characterized by impaired humoral immunity leading to increased susceptibility to Epstein-Barr virus infection and/or lymphoma. SAP has also been linked to autoimmune diseases such as systemic lupus erythematosus. Applications Reported: This XLP-1D12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This XLP-1D12 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear)...

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: SAP (SLAM-Associated Protein)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: XLP-1D12
• Concentration: 5 μL/Test
• Conjugate: eFluor 660
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Sh2d1a
• Gene Accession No.: O60880
• Gene Alias: DSHP; Duncan disease homolog; Duncan disease SH2-protein; Duncan's disease; EBVS; FLJ18687; FLJ92177; Gm686; HGNC:10820; IMD5; LYP; MTCP1; RGD1562408; RP5-1052M9.3; SAP; SAP/SH2D1A; SH2 domain containing 1A; SH2 domain protein 1A; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; SLAM-associated protein isoform SAP-2; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; Xlp; XLPD; XLPD1
• Gene Symbols: Sh2d1a
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4068
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Safety and Handling

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