Invitrogen™ SAP102 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1045

Catalog No. PA1045

Description

PA1-045 detects synapse-associated protein 102 (SAP102) from rat tissues. PA1-045 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~102 kDa protein representing SAP102 in rat brain homogenate. Immunohistochemical staining of SAP102 in rat hippocampus yields a pattern consistent with post-synaptic localization. The PA1-045 immunogen is a synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102. PA1-045 immunizing peptide (Cat. # PEP-023) is available for use in neutralization and control experiments.

Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three approximately 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Specifications

• Antigen: SAP102
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DLG3
• Gene Alias: discs large homolog 3; discs large MAGUK scaffold protein 3; discs, large homolog 3; discs, large homolog 3 (Drosophila); disks large homolog 3; DLG3; Dlgh3; KIAA1232; mKIAA1232; MRX; MRX90; NEDLG; Neuroendocrine-DLG; PPP1R82; protein phosphatase 1, regulatory subunit 82; PSD-95/SAP90-related protein 1; SAP102; SAP-102; synapse-associated protein 102; XLMR
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 58948
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q62936
• Gene Symbols: DLG3
• Immunogen: Synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG