Invitrogen™ SARS-CoV-2 NSP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5119597

Catalog No. PIPA5119597

Description

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Inhibits host translation by interacting with the 40S ribosomal subunit (PubMed:32680882). Nsp1 C terminus binds to and obstructs ribosomal mRNA entry tunnel (PubMed:32680882). Thereby inhibits antiviral response triggered by innate immunity or interferons (PubMed:32680882). The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

Specifications

• Antigen: SARS-CoV-2 NSP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: COVID19 Nsp1; COVID-19 Nsp1; SARS-CoV-2 Nonstructural Protein 1; SARS-CoV2 NSP1; SARS-COV-2 NSP1
• Immunogen: Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp1 (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG