Invitrogen™ Human EGF Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Specifications

• Accession Number: P01133
• For Use With (Application): ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): 6.3 kDa
• Name: Human EGF
• Quantity: 5 μg
• Source: E. coli
• Regulatory Status: RUO
• Gene Alias: AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
• Common Name: EGF
• Gene Symbol: EGF
• Biological Activity: 1.iPSC-derived human vascular organoids (Day 7) were cultured with FGF2, Human EGF Recombinant Protein. Red arrows represent vascular organoids. Image taken (at 10 x magnification.(Routinely tested) 2.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2 ng/mL)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Human EGF, amino acids Asn971-Arg1023 (Accession # NP_001954.2) with an N-terminal Met
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥95 % as determined by SDS-PAGE. ≥95 % as determined by SEC-HPLC.